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Definition 2024


shotgun_sequencing

shotgun sequencing

English

Noun

shotgun sequencing (uncountable)

  1. (genetics) A DNA sequencing technique in which a large number of small fragments of a long DNA strand are generated at random, sequenced, and reassembled to form a sequence of the original strand.
    • 2005, Selene K Swanson; Michael P. Washburn, “The continuing evolution of shotgun proteomics”, in Drug Discovery Today, page 719:
      In shotgun sequencing of genomic DNA, fragments of a genome are sequenced and computationally reassembled to determine the genome of an organism

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